Coding

Part:BBa_K2019003:Design

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-10-12)


Periplasm Directed saCas9 via TorA Signaling Sequence (TorA-saCas9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 753
    Illegal BglII site found at 906
    Illegal BglII site found at 1052
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal AgeI site found at 2882
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K1012002 was ordered as a gblock with homology to the BBa_K2019000-BBa_R0040 expression system. BBa_K2019000-BBa_R0040 was linearized at the 5' end of saCas9. A 2 piece gibson assembly was then performed with the gblock and Cas9 expression backbone.


Source

The TorA sequence is from part BBa_K1012002 of the 2013 Dundee Team. The saCas9 sequence is from another Northwestern 2016 iGEM part, BBa_K2019000.

References